During the winters of 2018 to 2020, בודי מסאז בתל אביב witches’ broom, phyllody, flat stem, little leaf, yellowing and stunting signs have been recorded in Mexican and French marigold fields in 4 states of India. The typical recorded disease incidence ranged from three to 30% in different states. 1.2 kb product of 16S rRNA gene was amplified in symptomatic marigold samples utilizing universal phytoplasma-specific nested primer pairs. Sequence comparison, phylogenetic and digital RFLP evaluation of 16S rRNA gene sequences identified the phytoplasmas in marigold samples as enclosed within the 16SrII-C subgroup for the strains from Delhi and within the 16SrI-B subgroup for the strains from Tripura, Uttar Pradesh and דירות דיסקרטיות ראשון לציון Maharashtra. Phytoplasma presence was further confirmed amplifying 4 genes (secA, rp, secY and tuf) in the symptomatic marigold species from all the surveyed locations. Moreover, six weed species rising in marigold fields resulted positive for phytoplasmas. Phyllanthus niruri (Delhi) was examined positive for 16SrII-C subgroup phytoplasma, nevertheless, Ageratum conyzoides, Acmella calva and Ludwigia perennis (Tripura), Cannabis sativa (Uttar Pradesh) and Parthenium hysterophorus (Maharashtra) were examined positive with 16SrI-B subgroup associated phytoplasma strains. Four leafhopper species had been additionally detected constructive for 16SrI-B subgroup collected from marigold fields at Tripura, Uttar Pradesh and Maharashtra and have been identified as putative vectors for recognized marigold phytoplasma strains.

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